Attachment Site Selection and Identity in Bxb1 Serine Integrase-Mediated Site-Specific Recombination
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چکیده
منابع مشابه
Attachment Site Selection and Identity in Bxb1 Serine Integrase-Mediated Site-Specific Recombination
Phage-encoded serine integrases mediate directionally regulated site-specific recombination between short attP and attB DNA sites without host factor requirements. These features make them attractive for genome engineering and synthetic genetics, although the basis for DNA site selection is poorly understood. Here we show that attP selection is determined through multiple proofreading steps tha...
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Vol. 40, No. 4 (2006) BioTechniques 1 Supplementary Figure S1. Structure of constructs used and representation of FRT locus. (A) Plasmids used to detect the Bxb1 integrase-mediated sitespecific recombination. CMVp, SV40t, Luc, stop, and Hygro are cytomegalovirus promoter (CMVp), simian virus 40 terminator (SV40t), luciferase gene, transcription termination sequence, and hygromycin resistance ge...
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ژورنال
عنوان ژورنال: PLoS Genetics
سال: 2013
ISSN: 1553-7404
DOI: 10.1371/journal.pgen.1003490